2ES4

Crystal structure of the Burkholderia glumae lipase-specific foldase in complex with its cognate lipase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lipase	polymer	319	33117.7	2	UniProt (Q05489) Pfam (PF00561) UniProt (by SIFTS) (P0DUB8) In PDB	Burkholderia glumae	Triacylglycerol lipase
2	D, E	Lipase chaperone	polymer	332	35236.2	2	UniProt (Q05490) Pfam (PF03280) In PDB	Burkholderia glumae	Lipase foldase, Lipase helper protein, Lipase activator protein, Lipase modulator
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	D, B, E	IODIDE ION	non-polymer		126.9	8	Chemie (IOD)
5		water	water		18.0	843	Chemie (HOH)

Sequence modifications

D, E: 22 - 332 (UniProt: Q05490)

PDB	External Database	Details
Gly 1	-	cloning artifact
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Ser 12	-	cloning artifact
Ser 13	-	cloning artifact
Gly 14	-	cloning artifact
His 15	-	cloning artifact
Ile 16	-	cloning artifact
Glu 17	-	cloning artifact
Gly 18	-	cloning artifact
Arg 19	-	cloning artifact
His 20	-	cloning artifact
Met 21	-	cloning artifact
Cso 92	Cys 112	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	136707.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1095.4
All*	Total formula weight	137803.3

*Water molecules are not included.