2EKE

Structure of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p: conservation of a noncovalent Ubiquitin-like protein-E2 complex as a platform for selective interactions within a SUMO pathway

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	SUMO-conjugating enzyme UBC9	polymer	157	17936.4	2	UniProt (P50623) Pfam (PF00179) In PDB	Saccharomyces cerevisiae (baker's yeast)	Ubiquitin-conjugating enzyme E2-18 kDa, Ubiquitin-protein ligase, Ubiquitin carrier protein 9
2	C, D	Ubiquitin-like protein SMT3	polymer	106	12190.7	2	UniProt (Q12306) Pfam (PF00240) In PDB	Saccharomyces cerevisiae (baker's yeast)
3		water	water		18.0	375	Chemie (HOH)

Sequence modifications

C, D: 1013 - 1098 (UniProt: Q12306)

PDB	External Database	Details
Met 93	-	initiating methionine
Gly 94	-	cloning artifact
Ser 95	-	cloning artifact
Ser 96	-	cloning artifact
His 97	-	expression tag
His 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
Ser 103	-	cloning artifact
Gln 104	-	cloning artifact
Asp 105	-	cloning artifact
Pro 106	-	cloning artifact
Leu 107	-	cloning artifact
Val 108	-	cloning artifact
Pro 109	-	cloning artifact
Arg 110	-	cloning artifact
Gly 111	-	cloning artifact
Ser 112	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	60254.2
All*	Total formula weight	60254.2

*Water molecules are not included.