2EJF

Crystal Structure Of The Biotin Protein Ligase (Mutations R48A and K111A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	235aa long hypothetical biotin--[acetyl-CoA-carboxylase] ligase	polymer	235	25959.3	2	UniProt (O57883) Pfam (PF03099) Pfam (PF02237) In PDB	Pyrococcus horikoshii	BPL
2	C, D	149aa long hypothetical methylmalonyl-CoA decarboxylase gamma chain	polymer	74	7985.5	2	UniProt (O59021) Pfam (PF00364) In PDB	Pyrococcus horikoshii	BCCP
3	A, B, C	BIOTIN	non-polymer		244.3	3	Chemie (BTN)
4	A, B	ADENOSINE	non-polymer		267.2	2	Chemie (ADN)
5	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6		water	water		18.0	409	Chemie (HOH)

Sequence modifications

A, B: 1 - 235 (UniProt: O57883)

PDB	External Database	Details
Ala 48	Arg 48	engineered mutation
Ala 111	Lys 111	engineered mutation

C, D: 77 - 149 (UniProt: O59021)

PDB	External Database	Details
Met 76	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	67889.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1359.5
All*	Total formula weight	69249.0

*Water molecules are not included.