2EJF
Crystal Structure Of The Biotin Protein Ligase (Mutations R48A and K111A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | 235aa long hypothetical biotin--[acetyl-CoA-carboxylase] ligase | polymer | 235 | 25959.3 | 2 | UniProt (O57883) Pfam (PF03099) Pfam (PF02237) | Pyrococcus horikoshii | BPL |
| 2 | C, D (C, D) | 149aa long hypothetical methylmalonyl-CoA decarboxylase gamma chain | polymer | 74 | 7985.5 | 2 | UniProt (O59021) Pfam (PF00364) | Pyrococcus horikoshii | BCCP |
| 3 | E, G, J (A, B, C) | BIOTIN | non-polymer | 244.3 | 3 | Chemie (BTN) | |||
| 4 | F, H (A, B) | ADENOSINE | non-polymer | 267.2 | 2 | Chemie (ADN) | |||
| 5 | I (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 409 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 235 (UniProt: O57883)
C, D: 77 - 149 (UniProt: O59021)
| PDB | External Database | Details |
|---|---|---|
| Ala 48 | Arg 48 | engineered mutation |
| Ala 111 | Lys 111 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 76 | - | initiating methionine |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 67889.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1359.5 | |
| All* | Total formula weight | 69249.0 |
