2E0O

Mutant Human Ribonuclease 1 (V52L, D53L, N56L, F59L)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ribonuclease	polymer	129	14705.7	2	UniProt (P07998) Pfam (PF00074) In PDB	Homo sapiens (human)	Ribonuclease 1, RNase 1, RNase A, RNase UpI-1, RIB-1, HP-RNase
2	A, B	SULFATE ION	non-polymer		96.1	9	Chemie (SO4)
3	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4		water	water		18.0	171	Chemie (HOH)

Sequence modifications

A, B: 1 - 128 (UniProt: P07998)

PDB	External Database	Details
Met 0	-	expression tag
Leu 52	Val 80	engineered mutation
Leu 53	Asp 81	engineered mutation
Leu 56	Asn 84	engineered mutation
Leu 59	Phe 87	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	29411.5
Non-Polymers*	Number of molecules	11
	Total formula weight	1048.8
All*	Total formula weight	30460.2

*Water molecules are not included.