2E08
Crystal structure of Glu140 to Lys mutant of Diphthine synthase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Probable diphthine synthase | polymer | 265 | 29613.4 | 2 | UniProt (O58456) Pfam (PF00590) | Pyrococcus horikoshii | Diphthamide biosynthesis methyltransferase |
| 2 | C, D, E, J, K... (A, B) | SULFATE ION | non-polymer | 96.1 | 7 | Chemie (SO4) | |||
| 3 | F (A) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
| 4 | G, N (A, B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) | |||
| 5 | H, I, O (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | P, Q (A, B) | water | water | 18.0 | 424 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 265 (UniProt: O58456)
| PDB | External Database | Details |
|---|---|---|
| Lys 140 | Glu 140 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 59226.9 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1723.6 | |
| All* | Total formula weight | 60950.5 |
