2DCZ

Thermal Stabilization of Bacillus subtilis Family-11 Xylanase By Directed Evolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Endo-1,4-beta-xylanase A	polymer	185	20454.1	2	UniProt (P18429) Pfam (PF00457) In PDB	Bacillus subtilis	Xylanase A, 1,4-beta-D-xylan xylanohydrolase A
2	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3	A	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	1	Chemie (DIO)
4		water	water		18.0	154	Chemie (HOH)

Sequence modifications

A, B: 1 - 185 (UniProt: P18429)

PDB	External Database	Details
His 7	Gln 35	engineered mutation
Phe 8	Asn 36	engineered mutation
Cys 179	Ser 207	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40908.3
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	41092.4

*Water molecules are not included.