2DCU

Crystal structure of translation initiation factor aIF2betagamma heterodimer with GDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Translation initiation factor 2 gamma subunit	polymer	419	46229.5	1	UniProt (Q8U082) Pfam (PF00009) Pfam (PF03144) Pfam (PF09173)	Pyrococcus furiosus	eIF-2-gamma, aIF2-gamma
2	B (B)	Translation initiation factor 2 beta subunit	polymer	148	17332.1	1	UniProt (Q8U3I5) Pfam (PF01873)	Pyrococcus furiosus	eIF-2-beta, aIF2-beta
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
4	D, F (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	E (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
6	G, H (A, B)	water	water		18.0	108	Chemie (HOH)

Sequence modifications

A: 1 - 411 (UniProt: Q8U082)

PDB	External Database	Details
Asp 236	Gly 236	engineered mutation
Leu 412	-	expression tag
Glu 413	-	expression tag
His 414	-	expression tag
His 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag

B: 1 - 140 (UniProt: Q8U3I5)

PDB	External Database	Details
Leu 141	-	expression tag
Glu 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	63561.5
Non-Polymers*	Number of molecules	4
	Total formula weight	598.3
All*	Total formula weight	64159.9

*Water molecules are not included.