2D0H
Crystal Structure of Thermoactinomyces vulgaris R-47 Alpha-Amylase 1 (TVAI) Mutant D356N/E396Q complexed with P2, a pullulan model oligosaccharide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | alpha-amylase I | polymer | 637 | 71108.3 | 1 | UniProt (Q60053) Pfam (PF02903) Pfam (PF00128) | Thermoactinomyces vulgaris | TVA I |
| 2 | B (B) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 990.9 | 1 | In PDB GlyTouCan (G84547LV) | |||
| 3 | C, D, E (A) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 4 | F (A) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 1 | Chemie (MPD) | |||
| 5 | G (A) | water | water | 18.0 | 383 | Chemie (HOH) |
Sequence modifications
A: 1 - 637 (UniProt: Q60053)
| PDB | External Database | Details |
|---|---|---|
| Asn 356 | Asp 385 | engineered mutation |
| Gln 396 | Glu 425 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 71108.3 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 990.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 238.4 | |
| All* | Total formula weight | 72337.6 |
