2CCL

THE S45A, T46A MUTANT OF THE TYPE I COHESIN-DOCKERIN COMPLEX FROM THE CELLULOSOME OF CLOSTRIDIUM THERMOCELLUM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	CELLULOSOMAL SCAFFOLDING PROTEIN A	polymer	158	16956.0	2	UniProt (Q06851) Pfam (PF00963)	CLOSTRIDIUM THERMOCELLUM	CELLULOSOMAL GLYCOPROTEIN S1/SL, CELLULOSE INTEGRATING PROTEIN A, COHESIN
2	B, D (B, D)	ENDO-1,4-BETA-XYLANASE Y	polymer	63	6794.9	2	UniProt (P51584) Pfam (PF00404)	CLOSTRIDIUM THERMOCELLUM	XYLANASE Y, XYLY, 1,4-BETA-D-XYLAN XYLANOHYDROLASE Y
3	E, F (A)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	G, H, I, J (B, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
5	K, L, M, N (A, B, C, D)	water	water		18.0	496	Chemie (HOH)

Sequence modifications

A, C: 1 - 2 (PDB: 2CCL)
A, C: 151 - 158 (PDB: 2CCL)
A, C: 3 - 150 (UniProt: Q06851)
B, D: -3 - -3 (PDB: 2CCL)
B, D: -2 - 59 (UniProt: P51584)

PDB	External Database	Details
Ala 45	Ser 777	engineered mutation
Ala 46	Thr 778	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	47501.8
Non-Polymers*	Number of molecules	6
	Total formula weight	350.3
All*	Total formula weight	47852.0

*Water molecules are not included.