2CBO

Crystal structure of the neocarzinostatin 3Tes24 mutant bound to testosterone hemisuccinate.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NEOCARZINOSTATIN	polymer	115	11615.6	1	UniProt (P0A3R9) Pfam (PF00960) In PDB	STREPTOMYCES CARZINOSTATICUS	NCS, MITOMALCIN, MMC
2	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3	A	TESTOSTERONE HEMISUCCINATE	non-polymer		388.5	2	Chemie (TH2)
4		water	water		18.0	116	Chemie (HOH)

Sequence modifications

A: 1 - 113 (UniProt: P0A3R9)

PDB	External Database	Details
Trp 33	Asp 67	engineered mutation
Ala 35	Gly 69	engineered mutation
Trp 37	Cys 71	engineered mutation
Arg 39	Trp 73	engineered mutation
Trp 45	Leu 79	engineered mutation
Tyr 47	Cys 81	engineered mutation
Asn 52	Phe 86	engineered mutation

A: 114 - 115 (PDB: 2CBO)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	11615.6
Non-Polymers*	Number of molecules	3
	Total formula weight	873.1
All*	Total formula weight	12488.6

*Water molecules are not included.