2BTM

DOES THE HIS12-LYS13 PAIR PLAY A ROLE IN THE ADAPTATION OF THERMOPHILIC TIMS TO HIGH TEMPERATURES?

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PROTEIN (TRIOSEPHOSPHATE ISOMERASE)	polymer	252	26981.6	2	UniProt (P00943) Pfam (PF00121)	Geobacillus stearothermophilus	TIM
2	C, D (A, B)	2-PHOSPHOGLYCOLIC ACID	non-polymer		156.0	2	Chemie (PGA)
3	E, F (A, B)	water	water		18.0	121	Chemie (HOH)

Sequence modifications

A, B: 1 - 251 (UniProt: P00943)

PDB	External Database	Details
Asn 12	His 13	engineered mutation
Gly 13	Lys 14	engineered mutation
Ala 230	Pro 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53963.3
Non-Polymers*	Number of molecules	2
	Total formula weight	312.1
All*	Total formula weight	54275.3

*Water molecules are not included.