2BG2
Bacillus cereus metallo-beta-lactamase (BcII) Arg (121) Cys mutant. Solved at pH4.5 using 20mM ZnSO4 in the buffer. 1mM DTT and 1mM TCEP- HCl were used as reducing agents. Cys221 is reduced.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | BETA-LACTAMASE II | polymer | 227 | 24941.5 | 2 | UniProt (P04190) Pfam (PF00753) | BACILLUS CEREUS | METALLO-BETA-LACTAMASE, PENICILLINASE, CEPHALOSPORINASE |
| 2 | C, D, E, F, G... (A, B) | GLYCEROL | non-polymer | 92.1 | 21 | Chemie (GOL) | |||
| 3 | AA, L, M, Z (B, A) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 4 | CA, DA, EA, FA, GA... (B, A) | SULFATE ION | non-polymer | 96.1 | 7 | Chemie (SO4) | |||
| 5 | BA (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | JA, KA (A, B) | water | water | 18.0 | 303 | Chemie (HOH) |
Sequence modifications
A, B: 209 - 211 (UniProt: P04190)
| PDB | External Database | Details |
|---|---|---|
| Cys 121 | Arg 121 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 49883.0 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 2903.5 | |
| All* | Total formula weight | 52786.5 |
