2BFL
Bacillus cereus metallo-beta-lactamase (BcII) Arg (121) Cys mutant. Solved at pH5 using 20mM ZnSO4 in buffer. 1mM DTT was used as a reducing agent.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Metallo-beta-lactamase type 2 | polymer | 227 | 24941.5 | 2 | UniProt (P04190) Pfam (PF00753) | Bacillus cereus | B2 metallo-beta-lactamase,Beta-lactamase II,Cephalosporinase,Metallo-beta-lactamase type II,Metallothioprotein beta-lactamase II,Penicillinase,Zinc-requiring beta-lactamase II |
| 2 | C, D, E, F, K... (A, B) | GLYCEROL | non-polymer | 92.1 | 10 | Chemie (GOL) | |||
| 3 | G, H, Q, R (A, B) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 4 | I, J, S, T, U (A, B) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 5 | N (B) | AZIDE ION | non-polymer | 42.0 | 1 | Chemie (AZI) | |||
| 6 | W, X (A, B) | water | water | 18.0 | 493 | Chemie (HOH) |
Sequence modifications
A, B: 27 - 291 (UniProt: P04190)
| PDB | External Database | Details |
|---|---|---|
| Cys 121 | Arg 121 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 49883.0 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 1704.9 | |
| All* | Total formula weight | 51587.9 |
