2BFC
Reactivity modulation of human branched-chain alpha-ketoacid dehydrogenase by an internal molecular switch
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | 2-OXOISOVALERATE DEHYDROGENASE ALPHA SUBUNIT | polymer | 400 | 45472.0 | 1 | UniProt (P12694) Pfam (PF00676) In PDB | HOMO SAPIENS (HUMAN) | BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE E1 COMPONENT ALPHA CHAIN, BCKDH E1-ALPHA, BCKDE1A |
| 2 | B | 2-OXOISOVALERATE DEHYDROGENASE BETA SUBUNIT | polymer | 342 | 37902.3 | 1 | UniProt (P21953) Pfam (PF02779) Pfam (PF02780) In PDB | HOMO SAPIENS (HUMAN) | BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE E1 COMPONENT BETA CHAIN, BCKDH E1-BETA |
| 3 | A, B | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 4 | A | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 5 | A | 2-{3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-4-METHYL-2-OXO-2,3-DIHYDRO-1,3-THIAZOL-5-YL}ETHYL TRIHYDROGEN DIPHOSPHATE | non-polymer | 440.3 | 1 | Chemie (TZD) | |||
| 6 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | water | water | 18.0 | 614 | Chemie (HOH) |
Sequence modifications
A: 1 - 400 (UniProt: P12694)
| PDB | External Database | Details |
|---|---|---|
| Phe 113 | Tyr 158 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 83374.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 665.5 | |
| All* | Total formula weight | 84039.8 |
