2B4U

Structure of the C252S mutant of Selenomonas ruminantium PTP-like phytase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	myo-inositol hexaphosphate phosphohydrolase	polymer	335	38740.7	2	UniProt (Q7WUJ1) Pfam (PF14566)	Selenomonas ruminantium
2	C, F (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	D (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	E, G (A, B)	MALONATE ION	non-polymer		102.0	2	Chemie (MLI)
5	H, I (A, B)	water	water		18.0	718	Chemie (HOH)

Sequence modifications

A, B: 34 - 346 (UniProt: Q7WUJ1)

PDB	External Database	Details
Met 13	-	expression tag
Gly 14	-	expression tag
Ser 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
Ser 23	-	expression tag
Ser 24	-	expression tag
Gly 25	-	expression tag
Leu 26	-	expression tag
Val 27	-	expression tag
Pro 28	-	expression tag
Arg 29	-	expression tag
Gly 30	-	expression tag
Ser 31	-	expression tag
His 32	-	expression tag
Met 33	-	expression tag
Ser 252	Cys 252	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77481.4
Non-Polymers*	Number of molecules	5
	Total formula weight	431.7
All*	Total formula weight	77913.0

*Water molecules are not included.