2B4P

Structure of the D223N mutant of Selenomonas ruminantium PTP-like phytase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	myo-inositol hexaphosphate phosphohydrolase	polymer	334	38361.2	2	UniProt (Q7WUJ1) Pfam (PF14566)	Selenomonas ruminantium
2	C, F (A, B)	MALONATE ION	non-polymer		102.0	2	Chemie (MLI)
3	D, E (B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	G, H (A, B)	water	water		18.0	610	Chemie (HOH)

Sequence modifications

A, B: 34 - 346 (UniProt: Q7WUJ1)

PDB	External Database	Details
Met 13	-	expression tag
Gly 14	-	expression tag
Ser 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
Ser 23	-	expression tag
Ser 24	-	expression tag
Gly 25	-	expression tag
Leu 26	-	expression tag
Val 27	-	expression tag
Pro 28	-	expression tag
Arg 29	-	expression tag
Gly 30	-	expression tag
Ser 31	-	expression tag
His 32	-	expression tag
Met 33	-	expression tag
Asn 223	Asp 223	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76722.5
Non-Polymers*	Number of molecules	4
	Total formula weight	275.0
All*	Total formula weight	76997.5

*Water molecules are not included.