2APO

Crystal Structure of the Methanococcus jannaschii Cbf5 Nop10 Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable tRNA pseudouridine synthase B	polymer	357	41087.6	1	UniProt (Q57612) Pfam (PF08068) Pfam (PF01509) Pfam (PF16198) Pfam (PF01472)	Methanocaldococcus jannaschii	tRNA pseudouridine 55 synthase, Psi55 synthase, tRNA-uridine isomerase, tRNA pseudouridylate synthase
2	B (B)	Ribosome biogenesis protein Nop10	polymer	60	7335.4	1	UniProt (P81303) Pfam (PF04135)	Methanocaldococcus jannaschii
3	C, D (A)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
4	E (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	F, G (A, B)	water	water		18.0	175	Chemie (HOH)

Sequence modifications

A: 1 - 336 (UniProt: Q57612)

PDB	External Database	Details
Met -20	-	cloning artifact
Gly -19	-	cloning artifact
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	cloning artifact
Ser -7	-	cloning artifact
Gly -6	-	cloning artifact
His -5	-	cloning artifact
Ile -4	-	cloning artifact
Glu -3	-	cloning artifact
Gly -2	-	cloning artifact
Arg -1	-	cloning artifact
His 0	-	cloning artifact

B: 401 - 460 (UniProt: P81303)

PDB	External Database	Details
Mse 401	Met 1	modified residue
Mse 404	Met 4	modified residue
Mse 406	Met 6	modified residue
Mse 448	Met 48	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48423.0
Non-Polymers*	Number of molecules	3
	Total formula weight	143.6
All*	Total formula weight	48566.6

*Water molecules are not included.