2AH9

Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Beta-1,4-galactosyltransferase 1	polymer	287	32773.2	3	UniProt (P15291) Pfam (PF13733) Pfam (PF02709) In PDB	Homo sapiens (human)	Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
2	D, E, F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		627.6	3	In PDB BIRD (PRD_900017) GlyTouCan (G47362BJ)		triacetyl-beta-chitotriose
3	C, A, B	6-AMINOHEXYL-URIDINE-C1,5'-DIPHOSPHATE	non-polymer		503.3	3	Chemie (UDH)
4	C, A, B	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
5	C, A, B	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
6	B, C, A	SULFATE ION	non-polymer		96.1	16	Chemie (SO4)
7	C, A	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	2	Chemie (DIO)
8	C	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
9		water	water		18.0	548	Chemie (HOH)

Sequence modifications

A, B, C: 126 - 398 (UniProt: P15291)

PDB	External Database	Details
Ala 112	-	SEE REMARK 999
Ser 113	-	SEE REMARK 999
Met 114	-	SEE REMARK 999
Thr 115	-	SEE REMARK 999
Gly 116	-	SEE REMARK 999
Gly 117	-	SEE REMARK 999
Gln 118	-	SEE REMARK 999
Gln 119	-	SEE REMARK 999
Met 120	-	SEE REMARK 999
Gly 121	-	SEE REMARK 999
Arg 122	-	SEE REMARK 999
Gly 123	-	SEE REMARK 999
Ser 124	-	SEE REMARK 999
Ala 125	-	SEE REMARK 999
Thr 337	Arg 336	engineered mutation
Thr 338	Cys 337	engineered mutation
His 340	Met 339	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	98319.7
Branched	Number of molecules	3
	Total formula weight	1882.8
Non-Polymers*	Number of molecules	32
	Total formula weight	4227.9
All*	Total formula weight	104430.4

*Water molecules are not included.