2A85
Crystal Structure of the G81A mutant of the Active Chimera of (S)-Mandelate Dehydrogenase in complex with its substrate 2-hydroxyoctanoate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | L(+)-mandelate dehydrogenase | polymer | 380 | 42146.3 | 1 | UniProt (P20932) UniProt (P05414) Pfam (PF01070) In PDB | Pseudomonas putida | S-mandelate dehydrogenase, MDH |
2 | A | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) | |||
3 | A | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
4 | A | (2S)-2-HYDROXYOCTANOIC ACID | non-polymer | 160.2 | 1 | Chemie (HOC) | |||
5 | water | water | 18.0 | 126 | Chemie (HOH) |
Sequence modifications
A: 1 - 176 (UniProt: P20932)
A: 197 - 374 (UniProt: P20932)
A: 177 - 196 (UniProt: P05414)
PDB | External Database | Details |
---|---|---|
Ala 81 | Gly 81 | engineered mutation |
His 375 | - | expression tag |
His 376 | - | expression tag |
His 377 | - | expression tag |
His 378 | - | expression tag |
His 379 | - | expression tag |
His 380 | - | expression tag |
A: 177 - 196 (UniProt: P05414)
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 42146.3 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 811.8 | |
All* | Total formula weight | 42958.1 |