2A7P

Crystal Structure of the G81A mutant of the Active Chimera of (S)-Mandelate Dehydrogenase in complex with its substrate 3-indolelactate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	(S)-Mandelate Dehydrogenase	polymer	380	42146.3	1	UniProt (P20932) UniProt (P05414) Pfam (PF01070)	Pseudomonas putida
2	B (A)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
3	C (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
4	D (A)	3-(INDOL-3-YL) LACTATE	non-polymer		205.2	1	Chemie (3IL)
5	E (A)	water	water		18.0	224	Chemie (HOH)

Sequence modifications

A: 1 - 176 (UniProt: P20932)

PDB	External Database	Details
Ala 81	Gly 81	engineered mutation
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag

A: 197 - 374 (UniProt: P20932)
A: 177 - 196 (UniProt: P05414)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42146.3
Non-Polymers*	Number of molecules	3
	Total formula weight	856.8
All*	Total formula weight	43003.1

*Water molecules are not included.