2A53

fluorescent protein asFP595, A143S, off-state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	GFP-like non-fluorescent chromoprotein FP595 chain 1	polymer	73	8067.2	2	UniProt (Q9GZ28)	Anemonia sulcata (snake-locks sea anemone)	asFP595
2	B, D (B, D)	GFP-like non-fluorescent chromoprotein FP595 chain 2	polymer	170	19173.7	2	UniProt (Q9GZ28) Pfam (PF01353)	Anemonia sulcata (snake-locks sea anemone)	asFP595
3	E (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	F, G, H, I (A, B, C, D)	water	water		18.0	577	Chemie (HOH)

Sequence modifications

A, C: 2 - 62 (UniProt: Q9GZ28)

PDB	External Database	Details
Met -10	-	expression tag
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

B, D: 65 - 232 (UniProt: Q9GZ28)

PDB	External Database	Details
Nrq 65	Met 63	chromophore
Nrq 65	Tyr 64	chromophore
Nrq 65	Gly 65	chromophore
Ser 143	Ala 143	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	54481.8
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	54517.3

*Water molecules are not included.