2A48

Crystal structure of amFP486 E150Q

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GFP-like fluorescent chromoprotein amFP486	polymer	240	26600.0	1	UniProt (Q9U6Y6)	Anemonia majano
2	B, C, D (A)	BETA-MERCAPTOETHANOL	non-polymer		78.1	3	Chemie (BME)
3	E (A)	water	water		18.0	72	Chemie (HOH)

Sequence modifications

A: 2 - 227 (UniProt: Q9U6Y6)

PDB	External Database	Details
Met -10	-	expression tag
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Cr7 68	Lys 68	chromophore
Cr7 68	Tyr 69	chromophore
Cr7 68	Gly 70	chromophore
Gln 150	Glu 150	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	26600.0
Non-Polymers*	Number of molecules	3
	Total formula weight	234.4
All*	Total formula weight	26834.4

*Water molecules are not included.