29NT
Structure of old yellow enzyme (Kluyveromyces marxianus) at 1.7 Angstroms resolution | Mutant Y200F
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Enoate reductase 1 | polymer | 417 | 45869.2 | 2 | UniProt (A0ABX6EPS5) | Kluyveromyces marxianus | |
| 2 | C, E (A, B) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) PubChem (643976) | |||
| 3 | D, F, G (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 4 | H, I (A, B) | water | water | 18.0 | 1137 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 404 (UniProt: A0ABX6EPS5)
| PDB | External Database | Details |
|---|---|---|
| Met -12 | - | initiating methionine |
| Gly -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Phe 200 | Tyr 200 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 91738.4 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1200.9 | |
| All* | Total formula weight | 92939.3 |
