28VY

sCMGE assembled on ARS1 DNA with Sld2 and RPA

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	DNA replication licensing factor MCM2	polymer	868	98911.5	1	UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (brewer's yeast)	Minichromosome maintenance protein 2
2	B (3)	DNA replication licensing factor MCM3	polymer	1006	111720.2	1	UniProt (P24279) Pfam (PF22207) Pfam (PF23191) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (brewer's yeast)	Minichromosome maintenance protein 3
3	C (4)	DNA replication licensing factor MCM4	polymer	933	105138.4	1	UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128)	Saccharomyces cerevisiae (brewer's yeast)	Cell division control protein 54
4	D (5)	Minichromosome maintenance protein 5	polymer	775	86505.7	1	UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933)	Saccharomyces cerevisiae (brewer's yeast)	Cell division control protein 46
5	E (6)	DNA replication licensing factor MCM6	polymer	1017	113110.2	1	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Saccharomyces cerevisiae (brewer's yeast)	Minichromosome maintenance protein 6
6	F (7)	DNA replication licensing factor MCM7	polymer	845	95049.9	1	UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF24901)	Saccharomyces cerevisiae (brewer's yeast)	Cell division control protein 47,Minichromosome maintenance protein 7
7	G, H (A, B)	DNA (5'-D(P*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*T)-3')	polymer	27	8316.4	2		synthetic construct
8	I (C)	DNA replication complex GINS protein PSF3	polymer	217	24464.9	1	UniProt (Q12146) Pfam (PF22466) Pfam (PF05916)	Saccharomyces cerevisiae (brewer's yeast)	Partner of Sld five 3
9	J (D)	DNA replication complex GINS protein SLD5	polymer	294	33983.6	1	UniProt (Q03406) Pfam (PF05916) Pfam (PF16922)	Saccharomyces cerevisiae (brewer's yeast)
10	K (E)	Cell division control protein 45	polymer	657	75154.7	1	UniProt (Q08032) Pfam (PF02724)	Saccharomyces cerevisiae (brewer's yeast)
11	L (F)	DNA polymerase epsilon subunit B	polymer	689	78425.9	1	UniProt (P24482) Pfam (PF04042)	Saccharomyces cerevisiae (brewer's yeast)	DNA polymerase II subunit 2
12	M (G)	DNA polymerase epsilon catalytic subunit A	polymer	2222	255992.5	1	UniProt (P21951) Pfam (PF03104) Pfam (PF22634) Pfam (PF08490) Pfam (PF23250) Pfam (PF22912)	Saccharomyces cerevisiae (brewer's yeast)	3'-5' exodeoxyribonuclease,DNA polymerase II subunit A
13	N (H)	DNA replication complex GINS protein PSF1	polymer	208	24230.6	1	UniProt (Q12488) Pfam (PF05916) Pfam (PF24997)	Saccharomyces cerevisiae (brewer's yeast)	Partner of Sld five 1
14	O (I)	DNA replication complex GINS protein PSF2	polymer	213	25096.8	1	UniProt (P40359) Pfam (PF25005) Pfam (PF05916)	Saccharomyces cerevisiae (brewer's yeast)	Partner of Sld five 2
15	AA, DA, P, S, U... (6, 7, 2, 3, 4...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG) PubChem (888)
16	CA, FA, GA, HA, Q... (6, 7, G, 2, 4...)	ZINC ION	non-polymer		65.4	7	Chemie (ZN)
17	R (2)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
18	BA, EA, T, V, Y (6, 7, 3, 4, 5)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	5	Chemie (ATP)

Sequence modifications

3: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

C: 1 - 194 (UniProt: Q12146)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Asn 45	Ser 45	variant

E: 1 - 167 (UniProt: Q08032)
E: 167 - 650 (UniProt: Q08032)

PDB	External Database	Details
Asp 167	-	linker
Tyr 167	-	linker
Lys 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Gly 167	-	linker
Asp 167	-	linker
Tyr 167	-	linker
Lys 167	-	linker
Asp 167	-	linker
Asp 167	-	linker
Asp 167	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	1144417.6
Non-Polymers*	Number of molecules	19
	Total formula weight	3566.8
All*	Total formula weight	1147984.4

*Water molecules are not included.