28SZ

Structure of mutated ASBT homologue from Leptospira biflexa in outward-facing form with Deoxycholate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putative transporter, sodium/bile acid transporter family protein	polymer	300	33292.6	1	UniProt (B0SJT7) Pfam (PF01758)	Leptospira biflexa
2	B, C (A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA) PubChem (923)
3	D (A)	(3ALPHA,5BETA,12ALPHA)-3,12-DIHYDROXYCHOLAN-24-OIC ACID	non-polymer		392.6	1	Chemie (DXC) PubChem (222528)
4	E (A)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A: 1 - 292 (UniProt: B0SJT7)

PDB	External Database	Details
Leu 14	Val 14	engineered mutation
Ala 83	Thr 83	engineered mutation
Pro 206	Thr 206	engineered mutation
Met 236	Ile 236	engineered mutation
Thr 239	Gly 239	engineered mutation
Gln 240	Pro 240	engineered mutation
Ser 243	Ile 243	engineered mutation
Ser 266	Ala 266	engineered mutation
Gln 269	Val 269	engineered mutation
Leu 270	Pro 270	engineered mutation
Gly 293	-	expression tag
Ser 294	-	expression tag
Leu 295	-	expression tag
Glu 296	-	expression tag
Val 297	-	expression tag
Leu 298	-	expression tag
Phe 299	-	expression tag
Gln 300	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33292.6
Non-Polymers*	Number of molecules	3
	Total formula weight	438.6
All*	Total formula weight	33731.2

*Water molecules are not included.