28OE
Human PRC1.4 in complex with native UBCH5C bound to a H3Kc27me3 mononucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isoform 2 of Polyhomeotic-like protein 2 | polymer | 327 | 36201.0 | 1 | UniProt (Q8IXK0) Pfam (PF21319) Pfam (PF16616) Pfam (PF00536) | Homo sapiens (human) | hPH2,Early development regulatory protein 2 |
| 2 | B (B) | Chromobox 7 | polymer | 162 | 18268.5 | 1 | UniProt (B0QYP2) Pfam (PF00385) Pfam (PF17218) | Homo sapiens (human) | |
| 3 | C (C) | E3 ubiquitin-protein ligase RING2 | polymer | 340 | 38062.7 | 1 | UniProt (Q99496) Pfam (PF13923) Pfam (PF16207) | Homo sapiens (human) | Huntingtin-interacting protein 2-interacting protein 3,HIP2-interacting protein 3,Protein DinG,RING finger protein 1B,RING1b,RING finger protein 2,RING finger protein BAP-1,RING-type E3 ubiquitin transferase RING2 |
| 4 | D (D) | Polycomb complex protein BMI-1 | polymer | 336 | 38024.2 | 1 | UniProt (P35226) Pfam (PF13923) Pfam (PF16207) | Homo sapiens (human) | Polycomb group RING finger protein 4,RING finger protein 51 |
| 5 | E (E) | Ubiquitin-conjugating enzyme E2 D3 | polymer | 147 | 16706.1 | 1 | UniProt (Q4R5N4) Pfam (PF00179) | Homo sapiens (human) | (E3-independent) E2 ubiquitin-conjugating enzyme D3,E2 ubiquitin-conjugating enzyme D3,Ubiquitin carrier protein D3,Ubiquitin-protein ligase D3 |
| 6 | F, J (F, J) | Histone H3 | polymer | 135 | 15271.9 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) | Xenopus | |
| 7 | G, K (G, K) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62798) | Xenopus | |
| 8 | H, L (H, L) | Histone H2A | polymer | 129 | 13978.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) | Xenopus | |
| 9 | I, M (I, M) | Histone H2B | polymer | 122 | 13524.8 | 2 | UniProt (A0A1B8Y854) | Xenopus | |
| 10 | N (N) | DNA (151-MER) | polymer | 248 | 76456.6 | 1 | Homo sapiens | ||
| 11 | O (O) | DNA (151-MER) | polymer | 248 | 76716.8 | 1 | Homo sapiens | ||
| 12 | P, Q, R, S (C, D) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) PubChem (32051) |
Sequence modifications
A: 1 - 323 (UniProt: Q8IXK0)
B: 1 - 158 (UniProt: B0QYP2)
C: 1 - 336 (UniProt: Q99496)
D: 1 - 326 (UniProt: P35226)
F, J: 1 - 135 (UniProt: A0A310TTQ1)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Glu 307 | Leu 307 | engineered mutation |
| Glu 311 | Leu 311 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 327 | - | expression tag |
| Ser 328 | - | expression tag |
| Gly 329 | - | expression tag |
| Ser 330 | - | expression tag |
| Leu 331 | - | expression tag |
| Glu 332 | - | expression tag |
| Val 333 | - | expression tag |
| Leu 334 | - | expression tag |
| Phe 335 | - | expression tag |
| Gln 336 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 110 | Cys 111 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 408512.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 408773.8 |
