28JQ

Poised Complex: BAM bound BepA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Outer membrane protein assembly factor BamA	polymer	790	88502.8	1	UniProt (A7ZHR7)	Escherichia coli
2	B (B)	Outer membrane protein assembly factor BamB	polymer	373	39882.4	1	UniProt (P77774)	Escherichia coli
3	C (C)	Outer membrane protein assembly factor BamC	polymer	320	34401.3	1	UniProt (P0A903)	Escherichia coli
4	D (D)	Outer membrane protein assembly factor BamD	polymer	226	25816.8	1	UniProt (P0AC02)	Escherichia coli
5	E (E)	Outer membrane protein assembly factor BamE	polymer	104	11610.8	1	UniProt (P0A937)	Escherichia coli
6	F (F)	Beta-barrel assembly-enhancing protease	polymer	492	54194.5	1	UniProt (P66948)	Escherichia coli
7	G (F)	ZINC ION	non-polymer		65.4	1	Chemie (ZN) PubChem (32051)

Sequence modifications

A: 21 - 810 (UniProt: A7ZHR7)

PDB	External Database	Details
Cys 211	Asp 211	engineered mutation

E: 20 - 113 (UniProt: P0A937)

PDB	External Database	Details
Gly 114	-	expression tag
Gly 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag

F: 28 - 487 (UniProt: P66948)

PDB	External Database	Details
Cys 155	Arg 155	engineered mutation
Gly 488	-	expression tag
Ser 489	-	expression tag
Ser 490	-	expression tag
Ala 491	-	expression tag
Trp 492	-	expression tag
Ser 493	-	expression tag
His 494	-	expression tag
Pro 495	-	expression tag
Gln 496	-	expression tag
Phe 497	-	expression tag
Glu 498	-	expression tag
Lys 499	-	expression tag
Gly 500	-	expression tag
Gly 501	-	expression tag
Gly 502	-	expression tag
Ser 503	-	expression tag
Gly 504	-	expression tag
Gly 505	-	expression tag
Gly 506	-	expression tag
Ser 507	-	expression tag
Gly 508	-	expression tag
Gly 509	-	expression tag
Ser 510	-	expression tag
Ala 511	-	expression tag
Trp 512	-	expression tag
Ser 513	-	expression tag
His 514	-	expression tag
Pro 515	-	expression tag
Gln 516	-	expression tag
Phe 517	-	expression tag
Glu 518	-	expression tag
Lys 519	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	254408.5
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	254473.9

*Water molecules are not included.