28JK

CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE-1 COMPLEXED WITH 3R-HYDROXY-4E-DECENOYL-CoA AND REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Peroxisomal bifunctional enzyme	polymer	742	80959.4	2	UniProt (P07896) Pfam (PF00378) Pfam (PF02737) Pfam (PF00725)	Rattus norvegicus (Norway rat)	PBE,PBFE,Multifunctional enzyme 1,MFE1,Multifunctional protein 1,MFP1
2	C, G (A, B)	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE	non-polymer		665.4	2	Chemie (NAI)
3	D, H (A, B)	(S)-3-HYDROXY-(E)-4-DECENOYL-COA	non-polymer		935.8	2	Chemie (A1JZ1)
4	E, F, I (A, B)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	J, K (A, B)	water	water		18.0	179	Chemie (HOH)

Sequence modifications

A, B: 1 - 722 (UniProt: P07896)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 514	Lys 514	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	161918.7
Non-Polymers*	Number of molecules	7
	Total formula weight	3490.6
All*	Total formula weight	165409.3

*Water molecules are not included.