28JJ

Crystal structure of rat peroxisomal multifunctional enzyme type-1 complexed with 2E,4E-decadienoyl-CoA, 3R-hydroxy-4E-decenoyl-CoA and NAD

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Peroxisomal bifunctional enzyme	polymer	742	80959.4	2	UniProt (P07896) Pfam (PF00378) Pfam (PF02737) Pfam (PF00725)	Rattus norvegicus (Norway rat)	PBE,PBFE,Multifunctional enzyme 1,MFE1,Multifunctional protein 1,MFP1
2	C, H (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
3	D (A)	2E,4E-decadienoyl-CoA	non-polymer		917.8	1	Chemie (A1JZN)
4	E, F, G (A)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	I (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
6	J (B)	(S)-3-HYDROXY-(E)-4-DECENOYL-COA	non-polymer		935.8	1	Chemie (A1JZ1)
7	K, L (A, B)	water	water		18.0	171	Chemie (HOH)

Sequence modifications

A, B: 1 - 722 (UniProt: P07896)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Arg 514	Lys 514	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	161918.7
Non-Polymers*	Number of molecules	8
	Total formula weight	3560.7
All*	Total formula weight	165479.4

*Water molecules are not included.