28FU
Cryo-EM structure of hydrogenase-2 in complex with L1 (right)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (E) | binder L1 | polymer | 82 | 8356.3 | 1 | synthetic construct | ||
| 2 | B, D (A, C) | Hydrogenase-2 small chain | polymer | 298 | 32371.5 | 2 | UniProt (P69741) | Escherichia coli (strain K12) | HYD2,Membrane-bound hydrogenase 2 small subunit,NiFe hydrogenase |
| 3 | C, E (B, D) | Hydrogenase-2 large chain | polymer | 567 | 62557.9 | 2 | UniProt (P0ACE0) | Escherichia coli (strain K12) | HYD2,Membrane-bound hydrogenase 2 large subunit,NiFe hydrogenase |
| 4 | F, H, K, M (A, C) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 4 | Chemie (SF4) | |||
| 5 | G, L (A, C) | FE3-S4 CLUSTER | non-polymer | 295.8 | 2 | Chemie (F3S) | |||
| 6 | I, N (B, D) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 2 | Chemie (FCO) | |||
| 7 | J, O (B, D) | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) PubChem (934) |
Sequence modifications
A, C: 2 - 293 (UniProt: P69741)
| PDB | External Database | Details |
|---|---|---|
| His 294 | - | expression tag |
| His 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 198215.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2387.3 | |
| All* | Total formula weight | 200602.5 |
