28FS

Cryo-EM structure of hydrogenase-2 in complex with L1 (left)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (E)	binder L1	polymer	82	8356.3	1		synthetic construct
2	B, D (A, C)	Hydrogenase-2 small chain	polymer	298	32371.5	2	UniProt (P69741)	Escherichia coli K-12	HYD2,Membrane-bound hydrogenase 2 small subunit,NiFe hydrogenase
3	C, E (B, D)	Hydrogenase-2 large chain	polymer	567	62557.9	2	UniProt (P0ACE0)	Escherichia coli K-12	HYD2,Membrane-bound hydrogenase 2 large subunit,NiFe hydrogenase
4	F, H, K, M (A, C)	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
5	G, L (A, C)	FE3-S4 CLUSTER	non-polymer		295.8	2	Chemie (F3S)
6	I, N (B, D)	CARBONMONOXIDE-(DICYANO) IRON	non-polymer		135.9	2	Chemie (FCO)
7	J, O (B, D)	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI) PubChem (934)

Sequence modifications

A, C: 2 - 293 (UniProt: P69741)

PDB	External Database	Details
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	198215.2
Non-Polymers*	Number of molecules	10
	Total formula weight	2387.3
All*	Total formula weight	200602.5

*Water molecules are not included.