27EM
Cryo_EM structure of PACAP27_PAC1R_Beta_arrestin 1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Beta-arrestin-1 | polymer | 391 | 43985.2 | 1 | UniProt (P49407) | Homo sapiens (human) | Arrestin beta-1,Non-visual arrestin-2 |
| 2 | B (C) | Fab30H | polymer | 230 | 24584.5 | 1 | synthetic construct | ||
| 3 | C (E) | Fab30L | polymer | 215 | 23435.1 | 1 | synthetic construct | ||
| 4 | D (P) | Pituitary adenylate cyclase-activating polypeptide 27 | polymer | 28 | 3152.7 | 1 | UniProt (P18509) | Homo sapiens | PACAP-27,PACAP27 |
| 5 | E (R) | Pituitary adenylate cyclase-activating polypeptide type I receptor | polymer | 443 | 51349.1 | 1 | UniProt (P41586) | Homo sapiens (human) | PAC1 receptor,PAC1R,PACAP type I receptor,PACAP-R-1,PACAP-R1 |
| 6 | F (P) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B: 4 - 393 (UniProt: P49407)
P: 1 - 27 (UniProt: P18509)
R: 21 - 415 (UniProt: P41586)
| PDB | External Database | Details |
|---|---|---|
| Met 3 | - | initiating methionine |
| Cys 279 | Ala 279 | conflict |
| Ala 386 | Ile 386 | conflict |
| Ala 387 | Val 387 | conflict |
| Ala 388 | Phe 388 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Nh2 28 | - | amidation |
| PDB | External Database | Details |
|---|---|---|
| Ala 415 | - | expression tag |
| Arg 415 | - | expression tag |
| Gly 415 | - | expression tag |
| Arg 415 | - | expression tag |
| Thr 415 | - | expression tag |
| Pro 415 | - | expression tag |
| Pro 415 | - | expression tag |
| Ser 416 | - | expression tag |
| Leu 416 | - | expression tag |
| Gly 416 | - | expression tag |
| Pro 416 | - | expression tag |
| Gln 416 | - | expression tag |
| Asp 416 | - | expression tag |
| Glu 416 | - | expression tag |
| Ser 416 | - | expression tag |
| Cys 432 | - | expression tag |
| Tpo 433 | - | expression tag |
| Tpo 434 | - | expression tag |
| Ala 435 | - | expression tag |
| Sep 436 | - | expression tag |
| Sep 437 | - | expression tag |
| Sep 438 | - | expression tag |
| Leu 439 | - | expression tag |
| Ala 440 | - | expression tag |
| Lys 441 | - | expression tag |
| Asp 442 | - | expression tag |
| Thr 443 | - | expression tag |
| Ser 444 | - | expression tag |
| Ser 445 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 146506.5 | |
| All* | Total formula weight | 146506.5 |
