25YJ

Cryo-EM structure of the V1 domain of V-ATPase from rat brain, state 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	V-type proton ATPase catalytic subunit A	polymer	647	71483.4	3	UniProt (D4A133) Pfam (PF02874) Pfam (PF16886) Pfam (PF00006) Pfam (PF22919)	Rattus norvegicus (Norway rat)	V-ATPase 69 kDa subunit,Vacuolar proton pump subunit alpha
2	D (H)	V-type proton ATPase subunit D	polymer	247	28359.0	1	UniProt (F7EUL0) Pfam (PF01813)	Rattus norvegicus (Norway rat)	V-type proton ATPase subunit d,Vacuolar proton pump subunit D
3	E, F, G (I, J, K)	V-type proton ATPase subunit E 1	polymer	226	26167.5	3	UniProt (Q6PCU2) Pfam (PF01991)	Rattus norvegicus (Norway rat)	V-ATPase subunit E 1,Vacuolar proton pump subunit E 1
4	H (L)	V-type proton ATPase subunit F	polymer	119	13389.3	1	UniProt (P50408) Pfam (PF01990)	Rattus norvegicus (Norway rat)	V-ATPase subunit F,V-ATPase 14 kDa subunit,Vacuolar proton pump subunit F
5	I, J, K (M, N, O)	V-type proton ATPase subunit G	polymer	118	13690.5	3	UniProt (Q8R2H0) Pfam (PF03179)	Rattus norvegicus (Norway rat)
6	L, M (Q, R)	Effector protein SidK	polymer	573	65505.3	2		Rattus norvegicus (Norway rat)
7	N, O, P (D, E, F)	V-type proton ATPase subunit B, brain isoform	polymer	511	56611.6	3	UniProt (P62815) Pfam (PF02874) Pfam (PF00006) Pfam (PF22919)	Rattus norvegicus (Norway rat)	V-ATPase subunit B 2,Endomembrane proton pump 58 kDa subunit,Vacuolar proton pump subunit B 2
8	Q (C)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
9	R (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	676617.7
Non-Polymers*	Number of molecules	2
	Total formula weight	451.5
All*	Total formula weight	677069.2

*Water molecules are not included.