25XN
Cryo-EM structure of the Crimean-Congo hemorrhagic fever virus full-length L protein RdRP elongation complex (EC14 form)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RNA-directed RNA polymerase L | polymer | 3989 | 453202.3 | 1 | UniProt (A0A068JD44) | Orthonairovirus haemorrhagiae | Large structural protein,Replicase,Transcriptase |
| 2 | B, F (B, F) | RNA (38-MER) | polymer | 38 | 12117.2 | 2 | synthetic construct | ||
| 3 | C (C) | RNA (5'-R(*GP*GP*AP*UP*AP*UP*AP*AP*UP*CP*AP*AP*AP*A)-3') | polymer | 14 | 4502.8 | 1 | synthetic construct | ||
| 4 | D (D) | RNA (5'-R(*UP*CP*UP*CP*AP*AP*AP*GP*AP*UP*AP*UP*CP*AP*AP*UP*CP*C)-3') | polymer | 18 | 5661.4 | 1 | synthetic construct | ||
| 5 | E (E) | RNA (5'-R(*GP*GP*GP*A)-3') | polymer | 4 | 1319.9 | 1 | synthetic construct | ||
| 6 | G, I, K (A) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) PubChem (32051) | |||
| 7 | H, J, L (A) | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) PubChem (27854) | |||
| 8 | M, N (A, B) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
A: 2 - 3945 (UniProt: A0A068JD44)
| PDB | External Database | Details |
|---|---|---|
| Met -43 | - | initiating methionine |
| Trp -42 | - | expression tag |
| Ser -41 | - | expression tag |
| His -40 | - | expression tag |
| Pro -39 | - | expression tag |
| Gln -38 | - | expression tag |
| Phe -37 | - | expression tag |
| Glu -36 | - | expression tag |
| Lys -35 | - | expression tag |
| Gly -34 | - | expression tag |
| Gly -33 | - | expression tag |
| Gly -32 | - | expression tag |
| Ser -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Gly -28 | - | expression tag |
| Ser -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Gly -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Trp -22 | - | expression tag |
| Ser -21 | - | expression tag |
| His -20 | - | expression tag |
| Pro -19 | - | expression tag |
| Gln -18 | - | expression tag |
| Phe -17 | - | expression tag |
| Glu -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ser 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 488920.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 361.0 | |
| All* | Total formula weight | 489281.8 |
