25TX

Cryo-EM structure of the 2:2 mGlu7-ELFN1 complex in conformation C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Metabotropic glutamate receptor 7	polymer	920	103128.6	2	UniProt (Q14831)	Homo sapiens (human)	mGluR7
2	C, D (C, D)	Protein ELFN1	polymer	411	45996.0	2	UniProt (P0C7U0)	Homo sapiens (human)	Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1,Protein phosphatase 1 regulatory subunit 28
3	E (E)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
4	F (F)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	1	In PDB GlyTouCan (G32152BH)
5	G, I (G, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
6	H (H)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
7	J (J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
8	K, L, M, N (A, B, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG) PubChem (24139)

Sequence modifications

A, B: 35 - 915 (UniProt: Q14831)

PDB	External Database	Details
Asp -4	-	expression tag
Tyr -3	-	expression tag
Lys -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Asp 1	-	expression tag
Asp 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Trp 5	-	expression tag
Ser 6	-	expression tag
His 7	-	expression tag
Pro 8	-	expression tag
Gln 9	-	expression tag
Phe 10	-	expression tag
Glu 11	-	expression tag
Lys 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Trp 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
Pro 20	-	expression tag
Gln 21	-	expression tag
Phe 22	-	expression tag
Glu 23	-	expression tag
Lys 24	-	expression tag
Gly 25	-	expression tag
Ser 26	-	expression tag
Ser 27	-	expression tag
Glu 28	-	expression tag
Asn 29	-	expression tag
Leu 30	-	expression tag
Tyr 31	-	expression tag
Phe 32	-	expression tag
Gln 33	-	expression tag
Ser 34	-	expression tag

C, D: 28 - 418 (UniProt: P0C7U0)

PDB	External Database	Details
Glu 419	-	expression tag
Phe 420	-	expression tag
Leu 421	-	expression tag
Glu 422	-	expression tag
Val 423	-	expression tag
Leu 424	-	expression tag
Phe 425	-	expression tag
Gln 426	-	expression tag
Gly 427	-	expression tag
Pro 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	298249.2
Branched	Number of molecules	6
	Total formula weight	3105.9
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	302239.9

*Water molecules are not included.