25SU

cryo-EM structure of human TAS2R4

Replaces:  9M8L

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Taste receptor type 2 member 4	polymer	365	40987.3	1	UniProt (Q9NYW5)	Homo sapiens (human)	T2R4
2	B (N)	Single-chain variable fragment 16	polymer	307	33013.0	1		Mus musculus
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-13	polymer	67	7959.3	1	UniProt (Q9P2W3)	Homo sapiens (human)
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3	polymer	351	38587.6	1	UniProt (P16520)	Homo sapiens (human)	Transducin beta chain 3
5	E (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(t) subunit alpha-3	polymer	239	27069.0	1	UniProt (P63096) UniProt (A8MTJ3)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein,Gustducin alpha-3 chain

Sequence modifications

R: 1 - 299 (UniProt: Q9NYW5)

PDB	External Database	Details
Met -27	-	initiating methionine
Lys -26	-	expression tag
Thr -25	-	expression tag
Ile -24	-	expression tag
Ile -23	-	expression tag
Ala -22	-	expression tag
Leu -21	-	expression tag
Ser -20	-	expression tag
Tyr -19	-	expression tag
Ile -18	-	expression tag
Phe -17	-	expression tag
Cys -16	-	expression tag
Leu -15	-	expression tag
Val -14	-	expression tag
Phe -13	-	expression tag
Ala -12	-	expression tag
Asp -11	-	expression tag
Tyr -10	-	expression tag
Lys -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Gly -4	-	expression tag
Ala -3	-	expression tag
Pro -2	-	expression tag
Ala -1	-	expression tag
Thr 0	-	expression tag
Glu 300	-	expression tag
Phe 301	-	expression tag
Leu 302	-	expression tag
Glu 303	-	expression tag
Val 304	-	expression tag
Leu 305	-	expression tag
Phe 306	-	expression tag
Gln 307	-	expression tag
Gly 308	-	expression tag
Pro 309	-	expression tag
Trp 310	-	expression tag
Ser 311	-	expression tag
His 312	-	expression tag
Pro 313	-	expression tag
Gln 314	-	expression tag
Phe 315	-	expression tag
Glu 316	-	expression tag
Lys 317	-	expression tag
Gly 318	-	expression tag
Gly 319	-	expression tag
Gly 320	-	expression tag
Ser 321	-	expression tag
Gly 322	-	expression tag
Gly 323	-	expression tag
Gly 324	-	expression tag
Ser 325	-	expression tag
Gly 326	-	expression tag
Gly 327	-	expression tag
Ser 328	-	expression tag
Ala 329	-	expression tag
Trp 330	-	expression tag
Ser 331	-	expression tag
His 332	-	expression tag
Pro 333	-	expression tag
Gln 334	-	expression tag
Phe 335	-	expression tag
Glu 336	-	expression tag
Lys 337	-	expression tag

B: 2 - 340 (UniProt: P16520)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

A: 1 - 57 (UniProt: P63096)

PDB	External Database	Details
Asp 42	Gly 42	conflict
Asn 43	Glu 43	conflict
Gly 58	-	linker
Gly 59	-	linker
Gly 60	-	linker
Gly 61	-	linker
Gly 62	-	linker
Gly 63	-	linker
Gly 64	-	linker
Gly 65	-	linker

A: 181 - 354 (UniProt: A8MTJ3)

PDB	External Database	Details
Asp 217	Gly 217	conflict
Asp 226	Ala 226	conflict
Ala 332	Val 332	conflict
Ile 335	Val 335	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	147616.1
All*	Total formula weight	147616.1

*Water molecules are not included.