25RZ

Cryo-EM structure of the E. coli beta sliding clamp-Hda heterohexamer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Beta sliding clamp	polymer	386	42801.9	4	UniProt (P0A990)	Escherichia coli	Beta clamp,Sliding clamp,Beta-clamp processivity factor,DNA polymerase III beta sliding clamp subunit,DNA polymerase III subunit beta
2	E, F (E, F)	DnaA regulatory inactivator Hda	polymer	233	26663.7	2	UniProt (P69931)	Escherichia coli	DnaA paralog,Dp,Protein IdaB
3	G, I (E, F)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
4	H, J (E, F)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)

Sequence modifications

A, B, C, D: 1 - 366 (UniProt: P0A990)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	224534.8
Non-Polymers*	Number of molecules	4
	Total formula weight	903.0
All*	Total formula weight	225437.8

*Water molecules are not included.