25MK

Cryo-EM structure of the Arabidopsis thaliana potassium transporter mutant - H527R, E565N, K759H, K778E, E734K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Potassium transporter 5	polymer	798	89456.4	2	UniProt (Q9M7K4)	Arabidopsis thaliana (thale cress)	AtHAK1,AtHAK5,AtPOT5
2	C, E (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
3	D, F (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K) PubChem (813)

Sequence modifications

A, B: 1 - 785 (UniProt: Q9M7K4)

PDB	External Database	Details
Met -12	-	initiating methionine
Asp -11	-	expression tag
Tyr -10	-	expression tag
Lys -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Lys -4	-	expression tag
Gly -3	-	expression tag
Cys -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Arg 527	His 527	engineered mutation
Asn 565	Glu 565	engineered mutation
Lys 734	Glu 734	engineered mutation
His 759	Lys 759	engineered mutation
Glu 778	Lys 778	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	178912.8
Non-Polymers*	Number of molecules	4
	Total formula weight	932.6
All*	Total formula weight	179845.4

*Water molecules are not included.