25LE

Cryo-EM structure of the Arabidopsis thaliana potassium transporter 5 mutant - H527A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Potassium transporter 5	polymer	798	89376.3	2	UniProt (Q9M7K4)	Arabidopsis thaliana (thale cress)	AtHAK1,AtHAK5,AtPOT5
2	C, E (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
3	D, F (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K) PubChem (813)
4	G, H (A, B)	water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B: 1 - 785 (UniProt: Q9M7K4)

PDB	External Database	Details
Met -12	-	initiating methionine
Asp -11	-	expression tag
Tyr -10	-	expression tag
Lys -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Lys -4	-	expression tag
Gly -3	-	expression tag
Cys -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Ala 527	His 527	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	178752.6
Non-Polymers*	Number of molecules	4
	Total formula weight	932.6
All*	Total formula weight	179685.2

*Water molecules are not included.