25IK

Cryo-EM structure of MasR(FL)-Gq

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Soluble cytochrome b562,Proto-oncogene Mas,LgBiT tag	polymer	620	70484.3	1	UniProt (P0ABE7) UniProt (P04201)	Escherichia coli	Cytochrome b-562
2	B (A)	Gs-mini-Gq chimera	polymer	246	28084.8	1		Homo sapiens
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	382	41772.6	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P63213)	Mus musculus (house mouse)	G gamma-I
5	E (S)	scFv16	polymer	248	26466.5	1		synthetic construct

Sequence modifications

R: -98 - 4 (UniProt: P0ABE7)

PDB	External Database	Details
Met -124	-	initiating methionine
Lys -123	-	expression tag
Thr -122	-	expression tag
Ile -121	-	expression tag
Ile -120	-	expression tag
Ala -119	-	expression tag
Leu -118	-	expression tag
Ser -117	-	expression tag
Tyr -116	-	expression tag
Ile -115	-	expression tag
Phe -114	-	expression tag
Cys -113	-	expression tag
Leu -112	-	expression tag
Val -111	-	expression tag
Phe -110	-	expression tag
Ala -109	-	expression tag
Asp -108	-	expression tag
Tyr -107	-	expression tag
Lys -106	-	expression tag
Asp -105	-	expression tag
Asp -104	-	expression tag
Asp -103	-	expression tag
Asp -102	-	expression tag
Lys -101	-	expression tag
Ala -100	-	expression tag
Asp -99	-	expression tag
Trp -94	Met 29	engineered mutation
Ile 1	His 124	engineered mutation
Leu 5	-	linker

R: 6 - 325 (UniProt: P04201)
R: 326 - 491 (PDB: 25IK)
B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Ser 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	174538.1
All*	Total formula weight	174538.1

*Water molecules are not included.