25EX

Cryo-EM structure of the Arabidopsis thaliana potassium transporter 5 in apo state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein,Potassium transporter 5	polymer	1153	129165.0	2	UniProt (P42212) UniProt (Q9M7K4)	Aequorea victoria (Water jellyfish Mesonema victoria)	AtHAK1,AtHAK5,AtPOT5
2	C, E (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
3	D, F (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K) PubChem (813)
4	G, H (A, B)	water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B: -253 - -17 (UniProt: P42212)

PDB	External Database	Details
Met -367	-	initiating methionine
Gly -366	-	expression tag
Gly -365	-	expression tag
Glu -364	-	expression tag
Gly -363	-	expression tag
Leu -362	-	expression tag
Arg -361	-	expression tag
Ala -360	-	expression tag
Ser -359	-	expression tag
Pro -358	-	expression tag
Arg -357	-	expression tag
Arg -356	-	expression tag
Arg -355	-	expression tag
Pro -354	-	expression tag
Leu -353	-	expression tag
Leu -352	-	expression tag
Pro -351	-	expression tag
Leu -350	-	expression tag
Gln -349	-	expression tag
Pro -348	-	expression tag
Arg -347	-	expression tag
Gly -346	-	expression tag
Cys -345	-	expression tag
Pro -344	-	expression tag
Arg -343	-	expression tag
Gly -342	-	expression tag
Asp -341	-	expression tag
Gly -340	-	expression tag
Cys -339	-	expression tag
Leu -338	-	expression tag
Arg -337	-	expression tag
Gly -336	-	expression tag
Gly -335	-	expression tag
Arg -334	-	expression tag
Gly -333	-	expression tag
Arg -332	-	expression tag
Ala -331	-	expression tag
Gly -330	-	expression tag
Phe -329	-	expression tag
Gly -328	-	expression tag
Phe -327	-	expression tag
Trp -326	-	expression tag
Arg -325	-	expression tag
Val -324	-	expression tag
Thr -323	-	expression tag
Gly -322	-	expression tag
Gly -321	-	expression tag
Ser -320	-	expression tag
Arg -319	-	expression tag
Ala -318	-	expression tag
Ser -317	-	expression tag
Ala -316	-	expression tag
Asn -315	-	expression tag
His -314	-	expression tag
Val -313	-	expression tag
His -312	-	expression tag
Ala -311	-	expression tag
Phe -310	-	expression tag
Phe -309	-	expression tag
Phe -308	-	expression tag
Phe -307	-	expression tag
Leu -306	-	expression tag
Gln -305	-	expression tag
Leu -304	-	expression tag
Leu -303	-	expression tag
Gly -302	-	expression tag
Asn -301	-	expression tag
Val -300	-	expression tag
Leu -299	-	expression tag
Val -298	-	expression tag
Ile -297	-	expression tag
Val -296	-	expression tag
Leu -295	-	expression tag
Ser -294	-	expression tag
His -293	-	expression tag
His -292	-	expression tag
Phe -291	-	expression tag
Gly -290	-	expression tag
Lys -289	-	expression tag
Glu -288	-	expression tag
Phe -287	-	expression tag
Ala -286	-	expression tag
Thr -285	-	expression tag
Met -284	-	expression tag
Ala -283	-	expression tag
His -282	-	expression tag
His -281	-	expression tag
His -280	-	expression tag
His -279	-	expression tag
His -278	-	expression tag
His -277	-	expression tag
His -276	-	expression tag
His -275	-	expression tag
Ala -274	-	expression tag
Ala -273	-	expression tag
Asp -272	-	expression tag
Tyr -271	-	expression tag
Lys -270	-	expression tag
Asp -269	-	expression tag
His -268	-	expression tag
Asp -267	-	expression tag
Ile -266	-	expression tag
Asp -265	-	expression tag
Tyr -264	-	expression tag
Lys -263	-	expression tag
Asp -262	-	expression tag
Asp -261	-	expression tag
Asp -260	-	expression tag
Asp -259	-	expression tag
Lys -258	-	expression tag
Ser -257	-	expression tag
Ala -256	-	expression tag
Met -255	-	expression tag
Val -254	-	expression tag
Leu -191	Phe 64	conflict
Thr -190	Ser 65	conflict
Thr -148	Lys 107	conflict
Lys -49	Ala 206	conflict
Leu -24	His 231	conflict
Ser -16	-	linker
Gly -15	-	linker
Leu -14	-	linker
Arg -13	-	linker
Ser -12	-	linker
Gly -11	-	linker
Leu -10	-	linker
Glu -9	-	linker
Val -8	-	linker
Leu -7	-	linker
Phe -6	-	linker
Gln -5	-	linker
Gly -4	-	linker
Pro -3	-	linker
Gly -2	-	linker
Gly -1	-	linker
Arg 0	-	linker

A, B: 1 - 785 (UniProt: Q9M7K4)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	258330.0
Non-Polymers*	Number of molecules	4
	Total formula weight	932.6
All*	Total formula weight	259262.6

*Water molecules are not included.