24XZ

P2Y14R-Gi complex bound to UDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	375	40586.3	1	UniProt (P62871)	Rattus rattus (black rat)	Transducin beta chain 1
2	B, D (G, A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	433	48846.7	2	UniProt (P63096)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (R)	Soluble cytochrome b562,P2Y purinoceptor 14,LgBiT tag,GFP	polymer	919	102814.0	1	UniProt (P0ABE7) UniProt (Q15391) UniProt (A0A5P9VSM6)	Escherichia coli	Cytochrome b-562,P2Y14,G-protein coupled receptor 105,UDP-glucose receptor
4	E (S)	scFv16	polymer	260	27720.8	1		Mus musculus
5	F (R)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP)

Sequence modifications

B: 2 - 340 (UniProt: P62871)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ala 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Gly 348	-	expression tag
Gly 349	-	expression tag
Asn 350	-	expression tag
Ser 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Ser 357	-	expression tag
Ser 358	-	expression tag
Gly 359	-	expression tag
Val 360	-	expression tag
Ser 361	-	expression tag
Gly 362	-	expression tag
Trp 363	-	expression tag
Arg 364	-	expression tag
Leu 365	-	expression tag
Phe 366	-	expression tag
Lys 367	-	expression tag
Lys 368	-	expression tag
Ile 369	-	expression tag
Ser 370	-	expression tag

G, A: 82 - 433 (UniProt: P63096)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Ser 3	-	expression tag
Asn 4	-	expression tag
Asn 5	-	expression tag
Thr 6	-	expression tag
Ala 7	-	expression tag
Ser 8	-	expression tag
Ile 9	-	expression tag
Ala 10	-	expression tag
Gln 11	-	expression tag
Ala 12	-	expression tag
Arg 13	-	expression tag
Lys 14	-	expression tag
Leu 15	-	expression tag
Val 16	-	expression tag
Glu 17	-	expression tag
Gln 18	-	expression tag
Leu 19	-	expression tag
Lys 20	-	expression tag
Met 21	-	expression tag
Glu 22	-	expression tag
Ala 23	-	expression tag
Asn 24	-	expression tag
Ile 25	-	expression tag
Asp 26	-	expression tag
Arg 27	-	expression tag
Ile 28	-	expression tag
Lys 29	-	expression tag
Val 30	-	expression tag
Ser 31	-	expression tag
Lys 32	-	expression tag
Ala 33	-	expression tag
Ala 34	-	expression tag
Ala 35	-	expression tag
Asp 36	-	expression tag
Leu 37	-	expression tag
Met 38	-	expression tag
Ala 39	-	expression tag
Tyr 40	-	expression tag
Cys 41	-	expression tag
Glu 42	-	expression tag
Ala 43	-	expression tag
His 44	-	expression tag
Ala 45	-	expression tag
Lys 46	-	expression tag
Glu 47	-	expression tag
Asp 48	-	expression tag
Pro 49	-	expression tag
Leu 50	-	expression tag
Leu 51	-	expression tag
Thr 52	-	expression tag
Pro 53	-	expression tag
Val 54	-	expression tag
Pro 55	-	expression tag
Ala 56	-	expression tag
Ser 57	-	expression tag
Glu 58	-	expression tag
Asn 59	-	expression tag
Pro 60	-	expression tag
Phe 61	-	expression tag
Arg 62	-	expression tag
Glu 63	-	expression tag
Lys 64	-	expression tag
Lys 65	-	expression tag
Phe 66	-	expression tag
Phe 67	-	expression tag
Cys 68	-	expression tag
Ala 69	-	expression tag
Ile 70	-	expression tag
Leu 71	-	expression tag
Gly 72	-	expression tag
Ser 73	-	expression tag
Ala 74	-	expression tag
Gly 75	-	expression tag
Ser 76	-	expression tag
Ala 77	-	expression tag
Gly 78	-	expression tag
Ser 79	-	expression tag
Ala 80	-	expression tag
Met 81	-	expression tag

R: -104 - 1 (UniProt: P0ABE7)

PDB	External Database	Details
Met -128	-	initiating methionine
Lys -127	-	expression tag
Thr -126	-	expression tag
Ile -125	-	expression tag
Ile -124	-	expression tag
Ala -123	-	expression tag
Leu -122	-	expression tag
Ser -121	-	expression tag
Tyr -120	-	expression tag
Ile -119	-	expression tag
Phe -118	-	expression tag
Cys -117	-	expression tag
Leu -116	-	expression tag
Val -115	-	expression tag
Phe -114	-	expression tag
Ala -113	-	expression tag
Asp -112	-	expression tag
Tyr -111	-	expression tag
Lys -110	-	expression tag
Asp -109	-	expression tag
Asp -108	-	expression tag
Asp -107	-	expression tag
Asp -106	-	expression tag
Lys -105	-	expression tag
Trp -98	Met 29	engineered mutation
Ile -3	His 124	engineered mutation
Leu 1	Arg 128	engineered mutation

R: 2 - 338 (UniProt: Q15391)

PDB	External Database	Details
Ser 510	-	linker
Gly 511	-	linker
Gly 512	-	linker
Ser 513	-	linker
Gly 514	-	linker
Gly 515	-	linker
Gly 516	-	linker
Gly 517	-	linker
Ser 518	-	linker
Gly 519	-	linker
Gly 520	-	linker
Ser 521	-	linker
Ser 522	-	linker
Ser 523	-	linker
Gly 524	-	linker
Gly 525	-	linker
Leu 526	-	linker
Glu 527	-	linker
Val 528	-	linker
Leu 529	-	linker
Phe 530	-	linker
Gln 531	-	linker
Gly 532	-	linker
Pro 533	-	linker
Gly 534	-	linker
Ser 535	-	linker
Ala 536	-	linker
Ala 537	-	linker
Ala 538	-	linker
Ala 539	-	linker

R: 339 - 509 (PDB: 24XZ)
R: 540 - 777 (UniProt: A0A5P9VSM6)

PDB	External Database	Details
Ser 778	-	expression tag
Gly 779	-	expression tag
Leu 780	-	expression tag
Arg 781	-	expression tag
Ser 782	-	expression tag
His 783	-	expression tag
His 784	-	expression tag
His 785	-	expression tag
His 786	-	expression tag
His 787	-	expression tag
His 788	-	expression tag
His 789	-	expression tag
His 790	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	268814.5
Non-Polymers*	Number of molecules	1
	Total formula weight	404.2
All*	Total formula weight	269218.7

*Water molecules are not included.