24XY

P2Y13R-Gq complex bound to ADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Soluble cytochrome b562,P2Y purinoceptor 13,LgBiT tag,GFP	polymer	915	102389.6	1	UniProt (P0ABE7) UniProt (Q9BPV8) UniProt (A0A5P9VSM6)	Escherichia coli	Cytochrome b-562,P2Y13,G-protein coupled receptor 86,G-protein coupled receptor 94
2	B (A)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2,miniGsqi	polymer	323	36260.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7547.7	1	UniProt (P63212)	Bos taurus (domestic cattle)	G gamma-I
5	E (N)	nanobody Nb35	polymer	137	15015.7	1		Lama glama
6	F (S)	scFv16	polymer	260	27720.8	1		Mus musculus
7	G (R)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

R: -84 - 21 (UniProt: P0ABE7)

PDB	External Database	Details
Met -108	-	initiating methionine
Lys -107	-	expression tag
Thr -106	-	expression tag
Ile -105	-	expression tag
Ile -104	-	expression tag
Ala -103	-	expression tag
Leu -102	-	expression tag
Ser -101	-	expression tag
Tyr -100	-	expression tag
Ile -99	-	expression tag
Phe -98	-	expression tag
Cys -97	-	expression tag
Leu -96	-	expression tag
Val -95	-	expression tag
Phe -94	-	expression tag
Ala -93	-	expression tag
Asp -92	-	expression tag
Tyr -91	-	expression tag
Lys -90	-	expression tag
Asp -89	-	expression tag
Asp -88	-	expression tag
Asp -87	-	expression tag
Asp -86	-	expression tag
Lys -85	-	expression tag
Trp -78	Met 29	engineered mutation
Ile 17	His 124	engineered mutation
Leu 21	Arg 128	engineered mutation

R: 22 - 354 (UniProt: Q9BPV8)

PDB	External Database	Details
Gly 355	-	linker
Ser 356	-	linker
Gly 357	-	linker
Gly 358	-	linker
Gly 359	-	linker
Gly 360	-	linker
Ser 361	-	linker
Gly 362	-	linker
Gly 363	-	linker
Ser 364	-	linker
Ser 365	-	linker
Ser 366	-	linker
Gly 367	-	linker
Gly 368	-	linker
Gly 527	-	linker
Gly 528	-	linker
Ser 529	-	linker
Gly 530	-	linker
Gly 531	-	linker
Gly 532	-	linker
Gly 533	-	linker
Ser 534	-	linker
Gly 535	-	linker
Gly 536	-	linker
Ser 537	-	linker
Ser 538	-	linker
Ser 539	-	linker
Gly 540	-	linker
Gly 541	-	linker
Leu 542	-	linker
Glu 543	-	linker
Val 544	-	linker
Leu 545	-	linker
Phe 546	-	linker
Gln 547	-	linker
Gly 548	-	linker
Pro 549	-	linker
Gly 550	-	linker
Ser 551	-	linker
Ala 552	-	linker
Ala 553	-	linker
Ala 554	-	linker
Ala 555	-	linker

R: 369 - 526 (PDB: 24XY)
R: 556 - 793 (UniProt: A0A5P9VSM6)

PDB	External Database	Details
Ser 794	-	expression tag
Gly 795	-	expression tag
Leu 796	-	expression tag
Arg 797	-	expression tag
Ser 798	-	expression tag
His 799	-	expression tag
His 800	-	expression tag
His 801	-	expression tag
His 802	-	expression tag
His 803	-	expression tag
His 804	-	expression tag
His 805	-	expression tag
His 806	-	expression tag

A: -76 - -10 (UniProt: P59768)

PDB	External Database	Details
Ser -9	-	linker
Gly -8	-	linker
Ser -7	-	linker
Ala -6	-	linker
Gly -5	-	linker
Ser -4	-	linker
Ala -3	-	linker
Gly -2	-	linker
Ser -1	-	linker
Ala 0	-	linker
Met 1	-	linker
Gly 2	-	linker
Ser 3	-	linker

A: 4 - 246 (PDB: 24XY)
B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
Ser 68	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	227678.3
Non-Polymers*	Number of molecules	1
	Total formula weight	427.2
All*	Total formula weight	228105.5

*Water molecules are not included.