24XB

Cryo-EM Structure of the Apo FFAR4-Gq Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	337	37069.5	1	UniProt (P62873)	Homo sapiens (human)
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7851.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
3	C (D)	scFv16	polymer	247	26337.3	1		Homo sapiens
4	D (E)	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	353	41381.1	1	UniProt (P50148)	Homo sapiens (human)	Guanine nucleotide-binding protein alpha-q
5	E (A)	Free fatty acid receptor 4	polymer	361	40533.2	1	UniProt (Q5NUL3)	Homo sapiens (human)	G-protein coupled receptor 120,G-protein coupled receptor 129,G-protein coupled receptor GT01,G-protein coupled receptor PGR4,Omega-3 fatty acid receptor 1

Sequence modifications

C: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Phe 62	Arg 62	conflict

E: 19 - 353 (UniProt: P50148)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Cys 3	-	expression tag
Thr 4	-	expression tag
Leu 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Val 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Ser 16	-	expression tag
Lys 17	-	expression tag
Met 18	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	153172.3
All*	Total formula weight	153172.3

*Water molecules are not included.