24WA

Cryo-EM structure of the human KCNQ2/KCNQ3 heterotetramer (1:3 stoichiometry) with CLM142 and PIP2 in open state (M2333 open, with symmetry expansion)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Potassium voltage-gated channel subfamily KQT member 3	polymer	620	71032.9	3	UniProt (O43525)	Homo sapiens (human)	KQT-like 3,Potassium channel subunit alpha KvLQT3,Voltage-gated potassium channel subunit Kv7.3
2	D (D)	Green fluorescent protein,Potassium voltage-gated channel subfamily KQT member 2	polymer	753	84657.3	1	UniProt (P42212) UniProt (O43526)	Aequorea victoria	KQT-like 2,Neuroblastoma-specific potassium channel subunit alpha KvLQT2,Voltage-gated potassium channel subunit Kv7.2
3	E, F, G, H (A, B, C, D)	~{N}-(7-cyclopropyl-1-prop-2-ynyl-indazol-3-yl)-4-fluoranyl-benzamide	non-polymer		333.4	4	Chemie (A1E7E)
4	I (D)	[(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate	non-polymer		746.6	1	Chemie (PIO)

Sequence modifications

A, B, C: 93 - 691 (UniProt: O43525)

PDB	External Database	Details
Met 72	-	initiating methionine
Ala 73	-	expression tag
Ser 74	-	expression tag
Asp 75	-	expression tag
Tyr 76	-	expression tag
Lys 77	-	expression tag
Asp 78	-	expression tag
His 79	-	expression tag
Asp 80	-	expression tag
Ile 81	-	expression tag
Asp 82	-	expression tag
Tyr 83	-	expression tag
Lys 84	-	expression tag
Asp 85	-	expression tag
Asp 86	-	expression tag
Asp 87	-	expression tag
Asp 88	-	expression tag
Lys 89	-	expression tag
Gly 90	-	expression tag
Thr 91	-	expression tag
Met 92	-	expression tag

D: -192 - 44 (UniProt: P42212)

PDB	External Database	Details
Met -208	-	initiating methionine
Ala -207	-	expression tag
Met -206	-	expression tag
His -205	-	expression tag
His -204	-	expression tag
His -203	-	expression tag
His -202	-	expression tag
His -201	-	expression tag
His -200	-	expression tag
His -199	-	expression tag
His -198	-	expression tag
Gly -197	-	expression tag
Gly -196	-	expression tag
Ser -195	-	expression tag
Met -194	-	expression tag
Val -193	-	expression tag
Leu -130	Phe 64	conflict
Thr -129	Ser 65	conflict
Thr -87	Lys 107	conflict
Lys 12	Ala 206	conflict
Leu 37	His 231	conflict
Ser 45	-	linker
Gly 46	-	linker
Leu 47	-	linker
Arg 48	-	linker
Ser 49	-	linker
Gly 50	-	linker
Leu 51	-	linker
Glu 52	-	linker
Val 53	-	linker
Leu 54	-	linker
Phe 55	-	linker
Gln 56	-	linker
Gly 57	-	linker
Pro 58	-	linker
Gly 59	-	linker
Gly 60	-	linker
Arg 61	-	linker
Met 62	-	linker

D: 63 - 351 (UniProt: O43526)
D: 360 - 528 (UniProt: O43526)

PDB	External Database	Details
Gly 352	-	linker
Gly 353	-	linker
Gly 354	-	linker
Ser 355	-	linker
Gly 356	-	linker
Gly 357	-	linker
Gly 358	-	linker
Ser 359	-	linker
Val 529	-	expression tag
Glu 530	-	expression tag
Gly 531	-	expression tag
Gly 532	-	expression tag
Ser 533	-	expression tag
Ser 534	-	expression tag
Gly 535	-	expression tag
Gly 536	-	expression tag
Trp 537	-	expression tag
Ser 538	-	expression tag
His 539	-	expression tag
Pro 540	-	expression tag
Gln 541	-	expression tag
Phe 542	-	expression tag
Glu 543	-	expression tag
Lys 544	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	297756.1
Non-Polymers*	Number of molecules	5
	Total formula weight	2080.0
All*	Total formula weight	299836.1

*Water molecules are not included.