24VL

Cryo-EM structure of the human wild-type KCNQ2/KCNQ3 heterotetramer (3:1 stoichiometry) in apo state (M2223 WT, without symmetry expansion)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Potassium voltage-gated channel subfamily KQT member 3	polymer	892	99199.8	1	UniProt (O43525)	Homo sapiens (human)	KQT-like 3,Potassium channel subunit alpha KvLQT3,Voltage-gated potassium channel subunit Kv7.3
2	B, C, D (B, C, D)	Green fluorescent protein,Potassium voltage-gated channel subfamily KQT member 2	polymer	1142	126318.7	3	UniProt (P42212) UniProt (O43526)	Aequorea victoria	KQT-like 2,Neuroblastoma-specific potassium channel subunit alpha KvLQT2,Voltage-gated potassium channel subunit Kv7.2
3	E, G, I (A, B, C)	(1R)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(heptanoyloxy)methyl]ethyl octadecanoate	non-polymer		593.8	3	Chemie (9PE)
4	F, H, J (B, C, D)	(2R)-2-{[(4-O-hexopyranosyl-beta-D-glucopyranosyl)oxy]methyl}-4-{[(25R)-5beta,14beta,17beta-spirostan-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside	non-polymer		1165.3	3	Chemie (YJ0)

Sequence modifications

A: 1 - 872 (UniProt: O43525)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
His -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

B, C, D: -254 - -18 (UniProt: P42212)

PDB	External Database	Details
Met -270	-	initiating methionine
Ala -269	-	expression tag
Met -268	-	expression tag
His -267	-	expression tag
His -266	-	expression tag
His -265	-	expression tag
His -264	-	expression tag
His -263	-	expression tag
His -262	-	expression tag
His -261	-	expression tag
His -260	-	expression tag
Gly -259	-	expression tag
Gly -258	-	expression tag
Ser -257	-	expression tag
Met -256	-	expression tag
Val -255	-	expression tag
Leu -192	Phe 64	conflict
Thr -191	Ser 65	conflict
Thr -149	Lys 107	conflict
Lys -50	Ala 206	conflict
Leu -25	His 231	conflict
Ser -17	-	linker
Gly -16	-	linker
Leu -15	-	linker
Arg -14	-	linker
Ser -13	-	linker
Gly -12	-	linker
Leu -11	-	linker
Glu -10	-	linker
Val -9	-	linker
Leu -8	-	linker
Phe -7	-	linker
Gln -6	-	linker
Gly -5	-	linker
Pro -4	-	linker
Gly -3	-	linker
Gly -2	-	linker
Arg -1	-	linker

B, C, D: 0 - 871 (UniProt: O43526)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	478156.0
Non-Polymers*	Number of molecules	6
	Total formula weight	5277.3
All*	Total formula weight	483433.3

*Water molecules are not included.