24TE

Crystal structure of GH30 Streptomyces avermitilis endo-beta-1,6-galactanase complexed with Gal-beta1,6-Gal

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Secreted endo-beta-1,6-galactanase	polymer	484	52293.7	1	UniProt (Q82CY3)	Streptomyces avermitilis MA-4680 = NBRC 14893
2	B, C (B, C)	beta-D-galactopyranose-(1-6)-beta-D-galactopyranose	branched		342.3	2	In PDB GlyTouCan (G08539FO)
3	D, E (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	F (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES) PubChem (4478249)
5	G (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
6	H (A)	water	water		18.0	224	Chemie (HOH)

Sequence modifications

A: 22 - 491 (UniProt: Q82CY3)

PDB	External Database	Details
Met 21	-	initiating methionine
Lys 492	-	expression tag
Leu 493	-	expression tag
Ala 494	-	expression tag
Ala 495	-	expression tag
Ala 496	-	expression tag
Leu 497	-	expression tag
Glu 498	-	expression tag
His 499	-	expression tag
His 500	-	expression tag
His 501	-	expression tag
His 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	52293.7
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	4
	Total formula weight	479.5
All*	Total formula weight	53457.8

*Water molecules are not included.