24PU

The crystal structure of the chicken FANCM-MHF1-MHF2(Q74C) disulfide-crosslinked complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Centromere protein S	polymer	108	11976.4	2	UniProt (E1BSW7) Pfam (PF15630)	Gallus gallus (chicken)	CENP-S
2	C (E)	Fanconi anemia group M protein	polymer	129	15418.2	1	UniProt (A0A1D5PRR9) Pfam (PF16783)	Gallus gallus (chicken)	Protein FACM,ATP-dependent RNA helicase FANCM,Fanconi anemia-associated polypeptide of 250 kDa,FAAP250,Protein Hef ortholog
3	D, E (C, D)	Centromere protein X	polymer	81	9336.8	2	UniProt (P0DJH7) Pfam (PF09415)	Gallus gallus (chicken)	CENP-X
4	F, G, H, I, J (A, B, E, C, D)	water	water		18.0	51	Chemie (HOH)

Sequence modifications

A, B: 2 - 106 (UniProt: E1BSW7)

PDB	External Database	Details
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 26	Cys 26	engineered mutation
Ala 28	Cys 28	engineered mutation
Ala 55	Cys 55	engineered mutation
Thr 107	-	expression tag

E: 680 - 804 (UniProt: A0A1D5PRR9)

PDB	External Database	Details
Gly 677	-	expression tag
Arg 678	-	expression tag
Ala 679	-	expression tag
Pro 805	-	expression tag

C, D: 2 - 80 (UniProt: P0DJH7)

PDB	External Database	Details
Gly 0	-	expression tag
Tyr 1	-	expression tag
Cys 74	Gln 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	58044.6
All*	Total formula weight	58044.6

*Water molecules are not included.