23ZR

Crystal structure of MonCI mutant N278R-S421A-Q441P-A253V

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	MonCI	polymer	511	55139.3	4	UniProt (Q846W9)	Streptomyces virginiae	Monensin epoxidase
2	E, F, G, H (A, B, C, D)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	4	Chemie (FAD)

Sequence modifications

A, B, C, D: 1 - 496 (UniProt: Q846W9)

PDB	External Database	Details
Met -14	-	initiating methionine
Asn -13	-	expression tag
His -12	-	expression tag
Lys -11	-	expression tag
Val -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Val 253	Ala 253	engineered mutation
Arg 278	Asn 278	engineered mutation
Ala 421	Ser 421	engineered mutation
Pro 441	Gln 441	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	220557.1
Non-Polymers*	Number of molecules	4
	Total formula weight	3142.2
All*	Total formula weight	223699.3

*Water molecules are not included.