23IL

Cryo-EM structure of Chaetomium thermophilum SWI/SNF bound to a nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3	polymer	135	15303.9	2	UniProt (A0A310TTQ1)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	125	13848.1	2	UniProt (A0A8J0U496)	Xenopus laevis (African clawed frog)
5	I (K)	WD40 repeat-containing protein	polymer	1595	179692.9	1	UniProt (G0SHD8)	Thermochaetoides thermophila DSM 1495	Snf2
6	J (L)	Putative chromatin structure remodeling complex protein	polymer	733	80291.3	1	UniProt (G0S882)	Thermochaetoides thermophila DSM 1495	Arp9
7	K (M)	Actin-related protein 4	polymer	476	52832.3	1	UniProt (G0SBW4)	Thermochaetoides thermophila DSM 1495
8	L, M (N, O)	Ssr1	polymer	694	75831.1	2	UniProt (G0S647)	Thermochaetoides thermophila DSM 1495
9	N (P)	DM2 domain-containing protein	polymer	547	61496.9	1	UniProt (G0RYN3)	Thermochaetoides thermophila DSM 1495	Ssr3
10	O (Q)	DUF1750-domain-containing protein	polymer	769	81947.3	1	UniProt (G0SEZ9)	Thermochaetoides thermophila DSM 1495	Ssr4
11	P (R)	ARID domain-containing protein	polymer	1301	140245.0	1	UniProt (G0S385)	Thermochaetoides thermophila DSM 1495	Sol1
12	Q (S)	Snf5	polymer	785	87782.3	1	UniProt (G0S4S8)	Thermochaetoides thermophila DSM 1495
13	R (T)	Nuclear localization protein	polymer	722	76208.4	1	UniProt (G0SEX4)	Thermochaetoides thermophila DSM 1495	Snf59
14	S (U)	LYR family protein	polymer	627	66726.4	1	UniProt (G0S145)	Thermochaetoides thermophila DSM 1495	Snf70
15	T (I)	DNA (167-MER)	polymer	167	51333.7	1		synthetic construct
16	U (J)	DNA (167-MER)	polymer	167	51774.0	1		synthetic construct
17	V (K)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

K: 3 - 1536 (UniProt: G0SHD8)

PDB	External Database	Details
Gly -58	-	expression tag
Ser -57	-	expression tag
Pro -56	-	expression tag
Gln -55	-	expression tag
Gln -54	-	expression tag
Asn -53	-	expression tag
Lys -52	-	expression tag
Thr -51	-	expression tag
Ala -50	-	expression tag
Ala -49	-	expression tag
Leu -48	-	expression tag
Ala -47	-	expression tag
Gln -46	-	expression tag
His -45	-	expression tag
Asp -44	-	expression tag
Glu -43	-	expression tag
Ala -42	-	expression tag
Asp -41	-	expression tag
Tyr -40	-	expression tag
Lys -39	-	expression tag
Asp -38	-	expression tag
His -37	-	expression tag
Asp -36	-	expression tag
Gly -35	-	expression tag
Asp -34	-	expression tag
Tyr -33	-	expression tag
Lys -32	-	expression tag
Asp -31	-	expression tag
His -30	-	expression tag
Asp -29	-	expression tag
Ile -28	-	expression tag
Asp -27	-	expression tag
Tyr -26	-	expression tag
Lys -25	-	expression tag
Asp -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Lys -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Glu -12	-	expression tag
Val -11	-	expression tag
Leu -10	-	expression tag
Phe -9	-	expression tag
Gln -8	-	expression tag
Gly -7	-	expression tag
Pro -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Thr 2	-	expression tag
Val 114	Ile 891	conflict
Ile 282	Val 1059	conflict
Thr 1513	Ala 2290	conflict

L: 1 - 711 (UniProt: G0S882)

PDB	External Database	Details
Pro 374	Ala 374	conflict
Ala 375	Thr 375	conflict
Lys 415	Arg 415	conflict
Ser 428	Pro 428	conflict
Glu 712	-	expression tag
Leu 713	-	expression tag
Gly 714	-	expression tag
Pro 715	-	expression tag
Lys 716	-	expression tag
Gly 717	-	expression tag
Ile 718	-	expression tag
Arg 719	-	expression tag
Ser 720	-	expression tag
His 721	-	expression tag
Ser 722	-	expression tag
Met 723	-	expression tag
Leu 724	-	expression tag
Ala 725	-	expression tag
Leu 726	-	expression tag
Glu 727	-	expression tag
His 728	-	expression tag
His 729	-	expression tag
His 730	-	expression tag
His 731	-	expression tag
His 732	-	expression tag
His 733	-	expression tag

M: 1 - 468 (UniProt: G0SBW4)

PDB	External Database	Details
Leu 469	-	expression tag
Glu 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag

N, O: 0 - 686 (UniProt: G0S647)

PDB	External Database	Details
Asp 687	-	expression tag
Tyr 688	-	expression tag
Lys 689	-	expression tag
Asp 690	-	expression tag
Asp 691	-	expression tag
Asp 692	-	expression tag
Asp 693	-	expression tag
Lys 694	-	expression tag

P: 1 - 539 (UniProt: G0RYN3)

PDB	External Database	Details
Asp 191	Asn 191	conflict
Asp 540	-	expression tag
Tyr 541	-	expression tag
Lys 542	-	expression tag
Asp 543	-	expression tag
Asp 544	-	expression tag
Asp 545	-	expression tag
Asp 546	-	expression tag
Lys 547	-	expression tag

Q: 2 - 762 (UniProt: G0SEZ9)

PDB	External Database	Details
Asp 763	-	expression tag
Tyr 764	-	expression tag
Lys 765	-	expression tag
Asp 766	-	expression tag
Asp 767	-	expression tag
Asp 768	-	expression tag
Asp 769	-	expression tag
Lys 770	-	expression tag

R: -7 - 1232 (UniProt: G0S385)

PDB	External Database	Details
Gly -68	-	expression tag
Ser -67	-	expression tag
Pro -66	-	expression tag
Gln -65	-	expression tag
Gln -64	-	expression tag
Asn -63	-	expression tag
Lys -62	-	expression tag
Thr -61	-	expression tag
Ala -60	-	expression tag
Ala -59	-	expression tag
Leu -58	-	expression tag
Ala -57	-	expression tag
Gln -56	-	expression tag
His -55	-	expression tag
Asp -54	-	expression tag
Glu -53	-	expression tag
Ala -52	-	expression tag
Asp -51	-	expression tag
Tyr -50	-	expression tag
Lys -49	-	expression tag
Asp -48	-	expression tag
His -47	-	expression tag
Asp -46	-	expression tag
Gly -45	-	expression tag
Asp -44	-	expression tag
Tyr -43	-	expression tag
Lys -42	-	expression tag
Asp -41	-	expression tag
His -40	-	expression tag
Asp -39	-	expression tag
Ile -38	-	expression tag
Asp -37	-	expression tag
Tyr -36	-	expression tag
Lys -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Asp -31	-	expression tag
Lys -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Ser -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Leu -23	-	expression tag
Glu -22	-	expression tag
Val -21	-	expression tag
Leu -20	-	expression tag
Phe -19	-	expression tag
Gln -18	-	expression tag
Gly -17	-	expression tag
Pro -16	-	expression tag
Gly -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Ala 397	Val 405	conflict
Gly 512	Val 520	conflict
Pro 632	Ser 640	conflict
Ile 724	Val 732	conflict
Ser 878	Gly 886	conflict
Ser 1036	Ala 1044	conflict

S: 1 - 777 (UniProt: G0S4S8)

PDB	External Database	Details
Asp 778	-	expression tag
Tyr 779	-	expression tag
Lys 780	-	expression tag
Asp 781	-	expression tag
Asp 782	-	expression tag
Asp 783	-	expression tag
Asp 784	-	expression tag
Lys 785	-	expression tag

T: -17 - 696 (UniProt: G0SEX4)

PDB	External Database	Details
Ser -7	Gly 11	conflict
Ser 682	Pro 700	conflict
Asp 697	-	expression tag
Tyr 698	-	expression tag
Lys 699	-	expression tag
Asp 700	-	expression tag
Asp 701	-	expression tag
Asp 702	-	expression tag
Asp 703	-	expression tag
Lys 704	-	expression tag

U: 1 - 608 (UniProt: G0S145)

PDB	External Database	Details
Ile 260	Val 260	conflict
Pro 459	Leu 459	conflict
Val 486	Ala 486	conflict
Asp 544	Gly 544	conflict
Glu 607	Gly 607	conflict
Ala 609	-	expression tag
Thr 610	-	expression tag
Gln 611	-	expression tag
Glu 612	-	expression tag
Val 613	-	expression tag
Lys 614	-	expression tag
Thr 615	-	expression tag
Glu 616	-	expression tag
Gly 617	-	expression tag
Glu 618	-	expression tag
Lys 619	-	expression tag
Pro 620	-	expression tag
Glu 621	-	expression tag
His 622	-	expression tag
His 623	-	expression tag
His 624	-	expression tag
His 625	-	expression tag
His 626	-	expression tag
His 627	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	21
	Total formula weight	1190779.5
Non-Polymers*	Number of molecules	1
	Total formula weight	427.2
All*	Total formula weight	1191206.7

*Water molecules are not included.